Target: 4-Hydroxynonenal
Conjugate: Unconjugated
Product Type: Monoclonal
Clone Number: 12F7
Immunogen: Synthetic 4-Hydroxynonenal modified Keyhole Limpet Hemocyanin (KLH).
Purification: Protein G Purified
Storage Buffer: PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated
Concentration: 1 mg/ml
Specificity: Specific for 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxynonenal. Does not cross-react with 4-Hydroxy-2-hexenal, Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
Scientific Background: 4-Hydroxy-2-nonenol (4-HNE) is an unsaturated aldehyde derived from lipid peroxidation. 4-HNE is an electrophile and reacts with protein nucleophiles such as cysteine, histine, and lysine (1). Low levels of 4-HNE promote cell survival via cellular antioxidant induction whereas higher levels lead to autophagy, apoptosis, and ultimately necrosis. 4-HNE has been linked to Alzheimer's disease, Parkinson's disease, cancer, cardiovascular diseases, diabetes, and liver disease.
References: 1. Peterson, D., Doorn, J. (2004) Free Radic Biol Med. 37(7):937-945.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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