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Encompassing Amino Acids: 2-790(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Reaction was performed in 50 mM Tris pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and various amount of A20 in a volume of 50 µL. Reaction was incubated at 37°C for 30 min. and fluorescent signal was measured at excitation of 360 nm, and emission at 460 nm.
Description: Human A20, also known as tumor necrosis factor-induced protein 3 (TNFAIP3) or OTU domain-containing protein 7C (OTUD7C), GenBank Accession No. NM_006290, a.a. 2-790(end) with an N-terminal FLAG-tag, MW=90.5 kDa, expressed in Sf9 insect cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 0.05% Tween-20, 20% glycerol, and 80 µg/ml FLAG peptide
Genbank: NM_006290
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal FLAG-tag
Uniprot: P21580
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Shi CS, Kehrl JH. (2010). Autophagy 6(7):986-7.
2. Tavares, R.M., et al. (2010). Immunity 33(2):181-9.
3. Hymowitz, S.G., Wertz, I.E. (2010). Nat. Rev. Cancer 10(5):332-41
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