A20, His-tag (E. coli-derived) Recombinant

A20, His-tag (E. coli-derived) Recombinant
SKU
BPS80393
Packaging Unit
100 µg
Manufacturer
BPS Bioscience

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Encompassing Amino Acids: 2-357

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and A20. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation = 340 nm, and emission = 460 nm.

Description: Human A20, also known as tumor necrosis factor-induced protein 3 (TNFAIP3) or OTU domain-containing protein 7C (OTUD7C), GenBank Accession No. NM_006290, a.a. 2-357 with an N-terminal His-tag, MW=42.8 kDa, expressed in an E. coli cell expression system.

Format: Aqueous buffer solution

Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 250 mM imidazole, and 20% glycerol

Genbank: NM_006290

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal His-tag

Uniprot: P21580

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Shi CS, Kehrl JH. (2010). Autophagy 6(7):986-7.
2. Tavares, R.M., et al. (2010). Immunity 33(2):181-9.
3. Hymowitz, S.G., Wertz, I.E. (2010). Nat. Rev. Cancer 10(5):332-41
More Information
SKU BPS80393
Manufacturer BPS Bioscience
Manufacturer SKU 80393
Green Labware No
Package Unit 100 µg
Quantity Unit STK
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