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Encompassing Amino Acids: 38-end
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: ABL2 (or ARG) is a nonreceptor cytoplasmic tyrosine kinase which is closely related to but distinct from ABL1. The similarity of ABL1 and ABL2 includes the tyrosine kinase domains and extends amino-terminal to include the SH2 and SH3 domains. ABL2 is involved in translocation with the ETV6 gene in human leukemia and has an altered expression in several human carcinomas (1). Two isoforms of ABL2 with different N-termini (1A and 1B) have been identified. The C-terminal domain of ABL2 contains two F-actin-binding sequences that perform a number of actions related to cell morphology and motility by interacting with actin filaments (2).
Description: Recombinant human ABL2 (a.a. 38-end), with N-terminal His-tag, expressed in Sf9 insect cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 50 mM sodium phosphate pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25% glycerol
Genbank: NM_005158
Host Cell Line: Sf9 cells
Purity: ≥75%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: P42684
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Barila, D. et al : An intramolecular SH3-domain interaction regulates c-Abl activity. Nature Genet. 18: 280-282, 1998.
2. Griesinger, F. et al: Identification of an ETV6-ABL2 fusion transcript in combination with an ETV6 point mutation in a T-cell acute lymphoblastic leukaemia cell line. Br J Haematol. 2002 Nov;119(2):454-8.
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