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Encompassing Amino Acids: 18-740
Applications: As an exopeptidase, ACE2 is useful for the study of enzyme kinetics and to screen for inhibitors. As a cell receptor for coronaviruses infection, including 2019-nCoV, ACE2 is useful for the study of protein-protein interactions.
Assay Conditions: Activity was determined using the Spike S1-Biotin (SARS-CoV-2): ACE2 TR-FRET Assay Kit (BPS Bioscience #79949). The ACE2 antibody was incubated with biotinylated Spike S1 (BPS Bioscience #100679), Eu-labeled ACE2 (BPS Bioscience #100705), and the dye-labeled acceptor for one hour. Then the TR-FRET signal was measured using a fluorescence reader.
Description: Human Angiotensin converting enzyme 2 (ACE2) also known ACEH, GenBank Accession No. NP_068576.1, a.a. 18-740, with a C-terminal His-tag, Eu-labeled, expressed in a HEK293 expression system. MW=85 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate, 110 mM NaCl, 2.2 mM KCl, 20% glycerol.
Genbank: NP_068576.1
Storage Stability: At least 6 months at -80°C.
Supplied As: Aqueous buffer solution.
Tags: C-terminal His-tag
Uniprot: Q9BYF1
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Keider, S., et al., Nephrology Dialysis Transplantation. 2007 22(2): 597-601.
2. Donoghue, M., et al., Circulation Research. 2002 Sep;87:e1-e9
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