ACE2: Spike S1 RBD, Mouse Fc-fusion (SARS-CoV-2) Inhibitor Screening Colorimetric Assay Kit

ACE2: Spike S1 RBD, Mouse Fc-fusion (SARS-CoV-2) Inhibitor Screening Colorimetric Assay Kit
SKU
BPS78031
Packaging Unit
96 rxns.
Manufacturer
BPS Bioscience

Availability: loading...
Price is loading...
Products from BPS Bioscience require a minimum order value above 400€

Applications: This kit is useful for screening for inhibitors of SARS-CoV-2 Spike RBD binding to ACE2.

Description: Coronavirus disease 2019 (COVID-19) increases the risk of developing Acute Respiratory Distress Syndrome (ARDS), which is often fatal at the late stages of the infection when the SAR-CoV-2 virus causes significant damage to the lungs. As a first step of the viral replication strategy, the virus attaches to the host cell surface before entering the cell. The Spike protein receptor binding domain (RBD) recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein of SARS-CoV-2 and ACE2 may offer some protection against the viral infection.The ACE2:SARS-CoV-2 Spike (RBD) Inhibitor Screening Colorimetric Assay Kit is designed for screening and profiling inhibitors of this interaction. The key to this kit is the high sensitivity of detection of Fc-tagged Spike protein by HRP-labeled Anti-mouse-Fc. Only a few simple steps on a microtiter plate are required for the assay. First, ACE2 protein is attached to a clear nickel-coated 96-well plate. Next, SARS-CoV-2 Spike-Fc is incubated with ACE2 on the plate. Finally, the plate is treated with HRP-labeled anti-Fc, followed by addition of an HRP substrate to produce color, which can then be measured using a UV/Vis spectrophotometer microplate reader.

Storage Stability: Up to 6 months from date of receipt, when stored as recommended.

Supplied As: This kit comes in a convenient 96-well format, with purified ACE2 and SARS-CoV-2 Spike proteins, HRP-labeled anti-mouse Fc region antibody, and assay buffer for 100 binding reactions.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: Hoffmann, M. et al. 2020. Cell, 181:1-10
Yan, R. et al. 2020. Science, 367(6485):1444-1448.
More Information
SKU BPS78031
Manufacturer BPS Bioscience
Manufacturer SKU 78031
Green Labware No
Package Unit 96 rxns.
Quantity Unit PAK
Product information (PDF) Download
MSDS (PDF)
×