Products from BPS Bioscience require a minimum order value above 400€Applications: Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput applications.
Background: Protein C, also known as autoprothrombin IIA and blood coagulation factor XIX, is a serine protease which acts as an anticoagulant and is normally expressed in its zymogen (pro-enzyme) form. Activated protein C (APC or PROC) inhibits coagulation by inactivating the proteolysis of Factor Va and Factor VIIIa and regulating blood vessel permeability and inflammatory responses. PROC activation is vitamin-K dependent and vitamin K deficiency or alterations in PROC activation can lead to increased risk of blood clot-related pathologies such as thrombosis.
Contraindications: The final concentration of DMSO in the assay should not exceed 1%.
Description: The Activated Protein C (APC, also known as PROC) Assay Kit is a 96-well format fluorogenic assay designed to measure the protease activity of Activated Protein C (PROC) for screening and profiling applications.
Illustration of the assay principle.
The substrate is an internally quenched fluorogenic substrate. Proteolysis releases the highly fluorescent AMC. Fluorescence intensity increases proportionally to the activity of the protease.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Supplied As: The kit contains sufficient amounts of purified recombinant PROC protein, PROC fluorogenic substrate, and PROC assay buffer for 100 enzyme reactions.
Uniprot: P04070
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
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