Products from BPS Bioscience require a minimum order value above 400€
Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Background: ADAM17 (ADAM Metallopeptidase Domain 17) is part of the ADAM family of disintegrins and metalloproteases. Initially identified as TNF-α converting enzyme (TACE), ADAM 17 has been linked to a number of diverse signaling pathways. It cleaves ectodomains of various transmembrane proteins and regulates cytokine shedding. It also plays a role in inflammatory skin and bowel disease.
Description: The ADAM17 Fluorescent Assay Kit is provided in a convenient 96-well format, with purified ADAM17, ADAM Fluorogenic Substrate, and ADAM assay buffer for 96 enzyme reactions. The key to the ADAM17 Fluorescent Assay Kit is the fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is required for ADAM17 reactions. A sample containing ADAM17 is incubated in a reaction mixture with the fluorogenic substrate and fluorescence (λex=485±10 nm, λem=530±10 nm) is measured using a plate reader.
Storage Stability: One year from date of receipt when stored as directed.
Biosafety Level: Not applicable (BSL-1)
References: 1. Scheller, Jürgen, et al. 2011. "ADAM17: a molecular switch to control inflammation and tissue regeneration." Trends in Immunology 32(8): 380-387.2. Blaydon, Diana C., et al. 2011. "Inflammatory skin and bowel disease linked to ADAM17 deletion." New England Journal of Medicine 365(16): 1502-1508.
English
Deutsch
