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Applications: Characterize the Fc effector function of antibodies and measure ADCC activity in cellular assays.
Description: The mouse ADCC Reporter cells (mFcγRIV /NFAT-Jurkat cells) are recombinant Jurkat T cell expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of mouse FcγRIV (NM_144559.2).
Format: Two vials containing ~ 2 x 106 cells in 1 ml of 10% DMSO in FBS.
Host Cell Line: Jurkat
Mycoplasma Testing: This cell line has been screened using the Venor™ GeM Mycoplasma Detection Kit, PCR Based (Sigma, #MP0025) to confirm the absence of Mycoplasma contamination.
Storage Stability: Store in liquid nitrogen immediately upon receipt. Do not store for long-term at -80°C or on dry ice.
Warnings: Avoid multiple freeze/thaw cycles.
Biosafety Level: BSL-1
References: Bhavin S. Parekh, et al. 2012. "Development and validation of an antibody-dependent cellmediated
cytotoxicity-reporter gene assay." mAbs, 4(3):310-318 , doi:10.4161/mabs.19873
Carlos Rosales. 2017. "FcγReceptor Heterogeneity in Leukocyte Functional Responses."
Frontiers in Immunology, 8: 280, doi:10.3389/fimmu.2017.00280
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