Application Note: Anti-Aha1 Antibody has been tested in western blot, ICC, and IHC and is suitable for use in ELISA, IF microscopy and IP. Expect a band approximately ~38kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
Concentration Value: 85 mg/mL
ELISA Dilution: 1:200
Immunohistochemistry Dilution: 1:1000
Immunoprecipitation Dilution: User Optimized
Western Blot Dilution: 0.5-1:1000
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen: Aha1 Antibody was produced from whole rabbit serum prepared by repeated immunizations with Mouse Aha1.
Physical State: Liquid (sterile filtered)
Purity and Specificity: Anti-Aha 1 Antibody was purified by Protein A chromatography. A BLAST analysis was used to suggest cross-reactivity with Aha 1 from Human, Mouse, and Rat based on 100% homology with the immunizing sequence. Cross-reactivity with Aha1 from other sources has not been determined. Heat Shock research.
Background: Aha1 is a member of the Hsp90 cochaperone family, and is thought to stimulate Hsp90 ATPase activity by competing with p23 and other co-chaperones for Hsp90 binding. It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/Hsp90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors.
Low Endotoxin: No
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