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Encompassing Amino Acids: 1-480(full length)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was done in Kinase buffer containing 1 mM DTT using Crosstide substrate (0.1 mg/ml) and 20 mM ATP. Reaction was done at 30°C for 40 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).
Description: Human AKT Serine/Threonine Kinase 1 (AKT1), also known as Protein Kinase B Alpha (PKBα), and RAC, GenBank Accession No. NM_001014432, a.a. 1-480(full length) with N-terminal His-tag, was expressed in Sf9 insect cells via a baculovirus expression system. MW=60 kDa. The purified AKT1 is activated in vitro using PDK1 enzyme.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, 200 mM Imidazole and 20% glycerol.
Genbank: NM_001014432
Host Cell Line: Sf9 cells
Purification: Purified by affinity chromatography
Storage Stability: At least 6 months at -80°C.
Supplied As: Aqueous buffer solution.
Tags: N-terminal His-tag
Uniprot: P31749
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Kuijl, C., et al., Nature. 2007 Nov 29; 450(7170):725-30.
2. Vogiatzi, P, and Giordano, A., Cancer Biol Ther. 2007 Aug 3; 6(10).Product Specific Reference(s):
1. Koeberle, A., et al., Proc Natl Acad Sci U S A. 2013 Feb 12; 110(7):2546-51.Application ReferencesPhosphorylation-Dependent Differences in CXCR4-LASP1-AKT1 Interaction between Breast Cancer and Chronic Myeloid Leukemia
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