Clone Name: 200000000
Light Chain: Kappa
Application Note: WB: 1:500-1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Calculated MW: 20
Specificity/Sensitivity: The clone 2E8 is specific for crystallin alpha B, but does not cross crystallin alpha A.
Form: Liquid
Buffer (with preservative): PBS, 10% Glycerol, 0.02% Sodium azide.
Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
Background: Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2019]
Uniprot ID: P02511
Antigen Species: Human
Immunogen: The clone 2E8 is derived from hybridization of mouse SP4/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant Human Crystallin alpha B.
Purification: Protein G Purified
Conjugation: Unconjugated
Full Name: crystallin alpha B
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