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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis (1). AMPK exists as a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits. Binding of AMP to the γ subunit allosterically activates the complex. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) (2) and via signaling pathways in response to adiponectin, leptin and CAMKKβ.
Description: Recombinant human AMPK (combination of A2/B2/G2 subunits), full length, with C-terminal His-tags, expressed in Sf9 insect cells via a baculovirus expression system. MW = 69 kDa (A2), 36 kDa (B2), 65 kDa (G2).
Format: Aqueous buffer solution
Formulation: 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.
Genbank: NM_006252 (A2), NM_005399 (B2), NM_001040633 (G2)
Host Cell Line: Sf9 cells
Purity: ≥75%
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal His-tags
Uniprot: A2: P54646; B2: O43741; G2: Q9UGJ0
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Hardie, G.D. The AMP-activated protein kinase pathway - new players upstream and downstream. J. Cell Sci. 2004;117: 5479-5487.
2. Kahn, B.B. et al. AMP-activated protein kinase: Ancient energy gauge provides clues to modern understanding of metabolism. Cell Metab; 2005: 1, 15-25.
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