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Encompassing Amino Acids: 1-460
Application: Useful for in vitro cell culture assays or in vivo applications.
Biological Activity: Angiopoietin-like 3 activity was confirmed by treating THP-1 cells with 500 ng/ml of angiopoietin-like 3 for 15 min, 30 min, 2 hr, or 4 hr. Phosphorylated-Erk was measured by Western blot and compared to total Erk levels.
Description: Human angiopoietin-like 3(ANGPTL3) also known as angiopoietin-relatedprotein 3, or angiopoietin 5 (Ang5),GenBank Accession No. NM_014495.3,a.a. 1-460 with a C-terminal FLAG tag,expressed in a HEK293 cell expressionsystem.
Endotoxin Level: <1 EU/µg
Format: Aqueous buffer solution
Formulation: Lyophilized powder from 8mM phosphate, pH 7.4, 100 mM NaCl, and2.2 mM KCl.
Genbank: NM_014495.3
Host Cell Line: HEK293
Purity: ≥90% as determined by SDS-PAGE
Storage Stability: >12 months at -80°C
Tags: C-terminal FLAG-tag
Uniprot: Q9Y5C1
Biosafety Level: Not applicable (BSL-1)
References: 1. Ando, Y., et al., J. Lipid Res. 44: 1216-1223.
2. Lee, E-Chiang, et al., J. Biol. Chem. 284:13735-13745.
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