CloneID: C219
Antigen Long Description: The original antibody was raised against plasma membranes of multidrug-resistant Chinese hamster ovary cells.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P21448
Specificity Statement: This antibody is specific for the epitope VQ(E/A)ALD of Chinese hamster ABCB1.
Application Notes (Clone): The original antibody was used for a western blot on whole cell extracts of E. coli cells overexpressing a fusion of the carboxyl-terminal NBD of Pgp to maltose-binding protein. The structure of the original antibody was determined using x-ray crystallography (Hoedemaeker et al., 1997; PMID:9368049, van Den Elsen et al., 1999; PMID:10570132). This antibody was used for immunohistochemistry on breast cancer cell lines, to determine the cross reactivity of this antibody with p185. This antibody was used for an ELISA on different peptides from p170 and p185 (Liu et al., 1997; PMID:9337349). This antibody was used for immnohistochemistry on high-grade lymphoma from dogs (Bergman et al., 1996; PMID:8947866), and on Ovarian Epithelium, Fallopian Tube, and Ovarian Carcinoma Specimens (Finstad et al., 1991; PMID:1682363).
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