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Applications: Useful for blocking NGF binding to its receptor in biochemical and cellular assays.
Assay Conditions: The Anti-NGF Antibody was evaluated using TrkA-SRE (Luc)- HEK293 cell line (BPS Bioscience #79798). NGF (500 pM at final concentration) was incubated with various concentrations of Anti-NGF Antibody in the assay medium (Opti-MEM) for 45 min. at room temperature and added to the cells. After 5 hour incubation at 37°C, ONE-Step™ Luciferase Assay System (BPS Bioscience #60690) was used to measure the luciferase activity.
Background: NGF is a neurotrophic factor (NTF) that is involved in growth, proliferation and survival of neurons. Anti-NGF monoclonal antibodies are in clinical trials as a chronic pain control medicine.
Cross Reactivity: This antibody has not been tested for cross-reactivity with any other species.
Description: Anti-NGF Neutralizing Recombinant Human antibody. This antibody has not been tested for crossreactivity with other species.
Format: Aqueous solution
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, pH 7.4, and 20% glycerol
Purification: Protein A affinity purificationfrom HEK293 supernatants.
Storage Stability: Stable for at least 12 months at -80°C.
Supplied As: Aqueous buffer solution.
Uniprot: P01138
Biosafety Level: Not applicable (BSL-1)
References: 1. Norman, B.H. et al., J. Med. Chem. 2017; 60(1): 66-88.
2. Lane, N.E., et al., N. Engl. J. Med. 2010; 363(16): 1521-1531.