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Applications: Useful for the detection of CD20 and binding to B cells or other cells expressing CD20 on their surface.
Assay Conditions: WIL2-S cells weretreated with anti-CD20 + 10% rabbitcomplement. Cell viability was determinedusing AlamarBlue® Indicator.
Description: Anti-CD20 is a recombinant monoclonal antibody which features the constant region of the human IgG1 isotype and the variable region of rituximab. It binds to human CD20 extracellular region a.a 142- 184, where it acts asan agonist of the CD20 protein. Itis expressed in a 293 freestyle cell expression system and purified using protein A chromatography. MW = 150 kDa.
Endotoxin Level: <1 EU/ µg
Format: Aqueous buffer solution
Formulation: 10 mM PBS pH 7.4, 138 mM NaCl, and 2.8 mM KCl.
Purification: Protein A affinitychromatography from HEK293supernatants.
Storage Stability: At least 6 months at -80°C.
Tags: none
Uniprot: P11836
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Ganjoo KN, et al., Leuk Lymphoma. 2014 April 9.2. Siegal T., J Clin Neurosci. 2014 April;21(5):709-715.
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