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Applications: This product is for research use only. It is not suitable for human diagnostic or therapeutic use.
Assay Conditions: Functional validation: The antibody was validated by ELISA using a purified CD20 antigen. The CD20 protein was coated onto a 96-well plate overnight at 4°C (50 µl/well at a concentration of 4 µg/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 µl of Blocking Buffer 2 (BPS Bioscience #79728) for 1 hour at room temperature. After removing the blocking buffer, 50 µl/well of purified biotinylated anti-CD20 antibody (BPS Bioscience #101207), serially diluted in Blocking Buffer 2, was added for 30 minutes at room temperature. After 3 more washes, the plate was incubated with Streptavidin-HRP, washed, and incubated with the Colorimetric HRP substrate. The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.
Description: Biotinylated anti-CD20 IgG antibody is a purified recombinant antibody which recognizes human CD20 antigen. This antibody has been tested for specific binding to purified human CD20 protein.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Purification: Protein A affinity
Purity: ≥90%
Storage Stability: At least 12 months at -80°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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