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Applications: The anti-CD235a IgG1 antibody can be used for flow cytometry and immunofluorescence microscopy.
Assay Conditions: See protocol for detailed information
Background: Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal sialoglycosylated segment, which lies outside the erythrocyte membrane, has MN and Ss blood group receptors.
Description: Anti-CD235a antibody is a purified biotinylated recombinant human antibody which recognizes the CD235a/GYPA protein. This antibody has been tested for specific binding to purified human CD235a protein (BPS Bioscience #101196) in a colorimetric ELISA binding assay. This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation is confirmed to be ≥90%.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2mM KCl, 20% glycerol
Purification: Protein A affinity purification from HEK293 cells
Purity: ≥90%
Storage Stability: At least 12 months at -80°C.
Tags: C-terminal Avi-Tag™
Uniprot: P02724
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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