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Products from BPS Bioscience require a minimum order value above 400€Application: Useful for studying expression of CD4 on the cell surface by flow cytometry.
Assay Conditions: One million PBMCs (BPS Bioscience #79059) were blocked and stained with 1 µg of anti-CD4 antibody, PE-labeled (BPS Bioscience #102010) or PE Human IgG1 Isotype Control (BioLegend #403503) for 30 minutes on ice, washed three times, and analyzed by flow cytometry. Non-lymphoid cells were excluded from the analysis based on side scatter and forward scatter signals. Y-axis represents the cell count while X-axis indicates the PE intensity.
Background: CD4 (cluster of differentiation 4) is part of the immunoglobulin superfamily, and is found in T-helper cells, monocytes, macrophages and dendritic cells. It is a co-receptor in the TCR (T-cell receptor), binding to MHC (major histocompatibility complex) class II molecules. CD4 binds to the tyrosine kinase Lck (lymphocyte-specific protein tyrosine kinase), which can phosphorylate the ITAM (immunoreceptor tyrosine activation motifs) domain of the CD3, activating CD3-related signaling. CD4 is a typical T cell marker of T helper cells, and it has been linked to cancer, autoimmune diseases such as vitiligo and type I diabetes. In addition, HIV-1 makes use of CD4 to trigger viral envelope protein conformational changes that allow cell infection. Ibalizumab, an anti-CD4 antibody, is currently used in the treatment of HIV, being considered a first-in-class medication. Further studies into CD4 and potential strategies around it may benefit patients and CD4 related diseases.
Cross Reactivity: This antibody recognizes human CD4. It has not been tested on other species.
Description: This anti-CD4 antibody is a purified recombinant antibody, which is labeled with R-Phycoerythrin (PE). This antibody has been tested by flow cytometry for specific binding to human CD4 on the surface of PBMCs (BPS Bioscience #79059).
Formulation: 8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, 0.09% Sodium Azide, 0.2% BSA, and up to 20% glycerol. May contain a protein stabilizer.
Host Cell Line: HEK293
Purification: Protein A affinity chromatography from HEK293 supernatants.
Purity: ≥90%
Storage Stability: At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE. The antibody solution should be stored undiluted between 2°C and 8°C.
Warnings: Do not freeze. Protect from light.
Biosafety Level: Not applicable (BSL-1)
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