CloneID: A54 scFv
Antigen Long Description: A54 human anti-CD40 scFv was selected from the n-CoDeR phage display library after 4 rounds of selections using biotinylated CD40-Fcγ fusion protein as bait and subsequent capture of bound phages on streptavidin-caoted magnetic beads.
Buffer Composition: PBS only.
Chimeric Use Statement: This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: P25942
Specificity Statement: A54 binds specifically to the D1 domain of CD40 (and can only bind to the intact, i.e., non-truncated version of CD40, and when an AD2 peptide tag has not been added) and can recognise CD40 on a B cell line (BJAB). The scFv version of this antibody binds CD40 (immobilized by amine coupling) with a KD of 40nM. CD40 is a glycoprotein of the TNFR superfamily and is expressed on all mature B cells, dendritic cells, activated monocytes, some endothelial cells, and some epithelium including the thymus. CD40 binds to the membrane protein CD154 of the TNF superfamily, and this interaction is required for the seconfary immune responses and germinal centre formation.
Application Notes (Clone): A54 is able to block the CD40-CD154 interaction by around 60-80% and can also induce CD40 sinalling in human B cells when at 'unusually high concentrations' (The CD40 receptor - target, tool and technology, Ellmark, 2002). This scFv can also be used for ELISA studies (PMID: 12153507)
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