CloneID: LAM1-116
Antigen Long Description: This original antibody was raised by immunizing a L-selectin deficient mouse with L-selectin.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P14151
Specificity Statement: This antibody is specific for the natural ligand binding domain of L-selectin.
Application Notes (Clone): The original antibody was used for flow cytometry on Holstein calve and sheep leukocytes (Leid et al., 2001; PMID:11290767), on EDTA-anticoagulated guinea pig blood (Fryer et al., 1997; PMID:9109449), an d on plasma samples from rats (Yan et al., 2000; PMID:10803628). This antibody was used for immunoprecipitation of L-selectin from bovine lymphocyte lysates (Leid et al., 2001; PMID:11290767), and on RBL cells (Haribabu et al., 1997; PMID:9153259). This antibody was used to test the effect of the antibody on NF-kappa B activation. It was shown that this antibody could indeed activate NF-kappa B in neutrophils (Turutin et al., 2003; PMID:12586371). This antibody was used for immunohistochemistry on a rat endometriosis model and on tissues of human endometriosis. This antibody was able to show the localization of the protein (Odagiri et al., 2007; PMID:17482182). This antibody was used for immunofluorescence on RBL cells where it showed staining like expected (Haribabu et al., 1997; PMID:9153259).
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