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Applications: Useful for studying CD8 expression on the cell surface by flow cytometry.
Assay Conditions: One million human PBMCs (BPS Bioscience #79059) were stained with 1 µg of anti-CD8 antibody, FITC-Labeled (BPS Bioscience #102224) or with FITC Human IgG1 Isotype Control (BioLegend #403507) for 30 minutes on ice, washed three times, and analyzed by flow cytometry. Non-lymphoid cells were excluded from the analysis based on side scatter and forward scatter signals (not shown). Y-axis represents the cell count, while X-axis shows FITC intensity.
Background: CD8 is a co-receptor for the TCR (T cell receptor) in T cells, binding to MHC (major histocompatibility complex) class I proteins. CD8 is a typical marker of cytotoxic T cells and is involved in cell signaling. CD8 exists as a disulfide-linked dimer of either α and β chains or two α chains. CD8 β recruits Lck (lymphocyte-specific protein tyrosine kinase) to the TCR-CD3 complex, and Lck phosphorylates multiple proteins involved in activation of cytotoxic T lymphocytes. It is thus critical for the effector function of cytotoxic T cells.
Cross Reactivity: This antibody recognizes human CD8. It has not been tested on other species.
Description: This anti-CD8 antibody is a purified recombinant antibody, which is labeled with R-Fluorescein isothiocyanate (FITC). This antibody has been tested by flow cytometry for specific binding to human CD8 on the surface of PBMCs (BPS Bioscience #79059).
Format: Aqueous buffer solution.
Formulation: 8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, 0.09% Sodium Azide, 0.2% BSA, and up to 20% glycerol. May contain a protein stabilizer.
Host Cell Line: HEK293
Purification: Protein A affinity chromatography from HEK293 supernatants.
Purity: ≥90%
Storage Stability: The antibody solution should be stored undiluted between 2°C and 8°C. At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE.
Uniprot: P01732
Biosafety Level: Not applicable (BSL-1)
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