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Products from BPS Bioscience require a minimum order value above 400€Application: Useful for studying expression of CD8 on the cell surface by flow cytometry.
Assay Conditions: One million PBMC cells (BPS Bioscience #79059) were blocked and stained with 1 µg of anti-CD8 antibody, PE-labeled (BPS Bioscience #102011) or PE Human IgG1 Isotype Control (BioLegend #403503) for 30 minutes on ice, washed three times, and analyzed by flow cytometry. Non-lymphoid cells were excluded from the analysis based on side scatter and forward scatter signals. Y-axis represents the cell count while X-axis indicates PE intensity.
Background: CD8 is a co-receptor for the TCR (T cell receptor) in T cells, binding to MHC (major histocompatibility complex) class I proteins. CD8 is a typical marker of cytotoxic T cells and is involved in signaling. CD8 has two isoforms, a and b. CD8b recruits Lck (lymphocyte-specific protein tyrosine kinase) to the TCR-CD3 complex, and Lck phosphorylates multiple proteins involved in activation of cytotoxic T lymphocytes. It is thus critical for the lysis of cancer cells.
Cross Reactivity: This antibody recognizes human CD8. It has not been tested on other species.
Description: This anti-CD8 antibody is a purified recombinant antibody, which is labeled with R-Phycoerythrin (PE). This antibody has been tested by flow cytometry for specific binding to human CD8 on the surface of PBMCs (BPS Bioscience #79059).
Format: Aqueous buffer solution.
Formulation: 8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, 0.09% Sodium Azide, 0.2% BSA, and up to 20% glycerol. May contain a protein stabilizer.
Host Cell Line: HEK293
Purification: Protein A affinity chromatography from HEK293 supernatants.
Purity: ≥90%
Storage Stability: At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE. The antibody solution should be stored undiluted between 2°C and 8°C.
Warnings: Do not freeze. Protect from light.
Biosafety Level: Not applicable (BSL-1)
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