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Applications: WB (1:500)
Assay Conditions: Western blot analysis using the antibody directed against CHD1
Western blot was performed on nuclear extracts from HeLa cells (HeLa nuclear extracts, 20 µg) with the antibody against CHD1 (Cat. No. 25213) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.
Background: CHD1 (UniProtKB/Swiss-Prot entry O14646) is an ATP-dependent chromatin remodeling factor, containing two tandem chromodomains. In human both chromodomains are essential for specific binding to histone H3, methylated at lysine 4. CHD1 belongs to the SNF2/RAD54 helicase family and contains also one helicase ATP-binding domain and one helicase C-terminal domain. It may play an important role in gene regulation.
Concentration: 50 µg/25 µl
Description: Polyclonal antibody raised in rabbit against human CHD1 (Chromodomain helicase DNA binding protein 1), using a GST-fused recombinant protein.
Format: Aqueous buffer solution
Formulation: PBS containing 0.05% azide and 0.05% ProClin 300.
Immunogen: recombinant protein
Purification: Affinity purified
Storage Stability: Store at -80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at -20°C for at least one month.
Uniprot: O14646
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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