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Applications: This product is for research use only. It is not suitable for human diagnostic or therapeutic use.
Assay Conditions: Functional validation: The antibody was validated by measuring its binding to CLDN18.2 antigen in ELISA. The CLDN18.2 protein was coated onto a 96-well plate overnight at 4°C (50 µl/well at a concentration of 2 µg/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 µl of Blocking Buffer 2 (BPS Bioscience #79728) with DDM/CHS detergent (dodecyl-β-D-maltoside (DDM) 0.05%, cholesteryl hemisuccinate (CHS) 0.01%) for 1 hour at room temperature. After removing the blocking buffer, 50 µl/well of purified anti-CLDN18.2 antibody (BPS Bioscience #101564), serially diluted in Blocking Buffer 2 with DDM/CHS detergent, was added for 60 minutes at room temperature. After 3 more washes, the plate was incubated with Goat Anti-Human IgG Fc (HRP) (Abcam #ab97225), washed, and incubated with Colorimetric HRP substrate (BPS Bioscience #79651). The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.
Description: Anti-Claudin-18 isoform 2 (CLDN18.2) IgG1 antibody is a purified recombinant antibody which recognizes human CLDN18.2 antigen. This antibody has been tested for specific binding affinity to purified human CLDN18.2 protein.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Host Cell Line: HEK293
Purification: Protein A affinity
Purity: ≥90%
Storage Stability: At least 12 months at -80°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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