CloneID: CTX-scFv8
Heavy Chain modification: His-Tagged
Antigen Long Description: scFv 8 was constructed from a phage display library using V-genes from 43 non-immunized donors.
Origin Pub PMID: 10736105
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This reformatted human antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P24027
Specificity Statement: scFv binds to crotoxin and recognises epitopes overlapping BI-a (an epitopic region at the interface between the CA and CB subunits of the heterodimer) and BIII (an epitopic region on CB involved in the enzymatic activity of CB) - scFv 8 may be able to caused the two subunits to dissociate and suppress the synergistic activity between them. Crotoxin is a phospholipase A2 neurotoxin and is the main toxic component in venome from Crotalus durissus terrificus rattlesnakes, acting by blocking neuromuscular transmission.
Application Notes (Clone): scFv 8 is able to reduce the myotoxicity induced by crotoxin and is able to neutralize the lethality induced by the toxin. This scFv was also used for (competitive ELISAs).
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