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Applications: WB, ICC, IP, ELISA
Clone Number: clone FG4R
Cross Reactivity: Works in human cells; predicted to work in all proteins containing the FLAG tag, regardless of species.
Description: Affinity purified Monoclonal Anti-FLAG-Tag, clone FG4R, mouse IgG2b. Recognizes FLAG-tagged (DYKDDDDK) proteins. Applications: Western Blotting (1:1000 - 3000), ELISA, Immunocytochemistry, immunoprecipitation, in situ hybridization (1:500 - 2000). Optimal dilutions/concentrations should be determined by the end user.
Format: Aqueous buffer solution
Formulation: 1 mg/ml in 10 mM PBS, pH 7.2, with 0.05% NaN3
Immunogen: Synthetic peptide: DYKDDDDK conjugated to KLH.
Purification: Protein A Affinity Chromatography
Storage Stability: Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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