Anti-Glycoprotein H (A13-d6.3)

Anti-Glycoprotein H [A13-d6.3], Recombinant, Fab fragment kappa, Human

CloneID: A13-d6.3

Heavy Chain modification: His-Tagged

Antigen Long Description: d6.3 was generated by immunizing mice with PrV.

Buffer Composition: PBS only.

Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Uniprot Accession No.: A0A142I9U7

Specificity Statement: d6.3 Fab fragment binds to the core fragment of the PrV glycoprotein H (gH) ectodomain (aa 107–639) which is the membrane-proximal end of the protein in the ΔgL-Pass PrV variant of PrV. The antibody contacts residues 332-373 (peripheral helices α6 and α7 of domain III of gH contribute most of the residues that form the epitope recognized by d6.3). PrV is a porcine herpesvirus belonging to the alpha-Herpesvirinae subfamily and is known to cause Aukeszky's disease in pigs. gH is a type I membrane portein and can interact non-covalently with gL via the large gH ectodomain.

Application Notes (Clone): d6.3 neutralizes the ΔgL-Pass PrV variant of PrV and can stain ΔgL-Pass PrV variant but not the wild-type PrV by immunostaining. The epitope of d6.3 is not blocked by the formation of the gH/gL heterodimer. The d6.3Fab fragment (obtained through papain digestion) can be complexed with the core fragment of the PrV glycoprotein H (gH) ectodomain (aa 107–639) for structural studies by crystallography.

• SKU: ABAAb00772-10.6-BT
• Manufacturer: Absolute Antibody
• Manufacturer SKU: Ab00772-10.6-BT
• Package Unit: 500 μg
• Quantity Unit: STK
• Reactivity: Virus
• Clonality: Recombinant
• Application: Neutralization
• Isotype: Fab fragment kappa
• Host: Human