CloneID: 5C3
Antigen Long Description: This antibody was prepared by immunizing BALB/c mice with a highly purified preparation of HBsAg.
Buffer Composition: PBS only.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: Q773S4
Specificity Statement: This antibody is specific for hepatitis B virus surface antigen, a common antigen on the surface of HBV-infected hepatocytes and the outer coat of the intact virus. It recognizes all known subtypes of HBsAg, and has a very high affinity constant for its HBsAg determinant. This antibody does not cross-react with 2C6 and 5D3, two other anti-HBsAg Mabs, as each recognizes a distinct and separate determinant on HBsAg.
Application Notes (Clone): This antibody, when radio-labelled with iodine-125, has been used to develop highly sensitive solid-phase radioimmunoassays for the detection of HBsAg at subnanogram levels in human serum (Shafritz, 1982). Consequently, this antibody may be a useful reagent in the immunodiagnosis of hepatitis B infection.
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