CloneID: F124
Antigen Long Description: BALB/c mice were immunized by injection of HBV particles serotype ay, purified from HBsAg-positive patient blood sera. Splenocytes from immunized mice were then fused with the Sp2/0-Ag myeloma cell line.
Origin Pub PMID: 9891981
Buffer Composition: PBS only.
Uniprot Accession No.: Q773S4
Specificity Statement: F124 was shown to react with an epitope within the pre-S2 region of the HBV surface antigen M protein, shown by Western blot and RIA using both full viral particles and HBsAg particles (Budkowska et al, 1986). F124 is also reactive against a synthetic peptide corresponding to the N-terminal residues 120-150 (FPAGGSSSGTVNPVLTTASPL) of the pre-S2 region (Ni et al, 2010). Hepatitis B is one of the most common infectious diseases on a global scale, with infection associated with both acute and chronic liver inflammation and implicated in up to half of all occurring hepatocellular carcinomas.
Application Notes (Clone): F124 was used as a solid-phase coating for RIAs detecting HBV particles, resulting in particle immobilisation by binding the pre-S2 epitope of the surface antigen (Budkowska et al, 1986). An scFv-formatted version of F124, comprising the same VH and VL sequences, showed dose-dependent binding to, and competitive blocking of IgG and Fab against, r-HBsAg in an ELISA (Passafiume et al, 1998). Size-exclusion HPLC indicates that, while the scFv monomer shows no tendency to aggregate at low concentrations, equilibrium is achieved at a monomer/dimer ratio of 3:1 at high protein concentrations.
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