Products from BPS Bioscience require a minimum order value above 400€
Applications: WB, DB, ELISA, ICC, EMSA, IP
Background: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Clone Number: clone HIS.H8/ EH158
Cross Reactivity: Works in human cells; predicted to work in all proteins containing the 6His motif, regardless of species.
Description: Affinity purified monoclonal Anti-His-Tag, clone HIS.H8/ EH158, mouse IgG2b. Recognizes His-tagged recombinant proteins or His-tagged proteins overexpressed in cells. Applications: Western blotting (1:1000), ICC (1:200), IP (1:200), ELISA, Electrophoretic mobility shift assay. Optimal dilutions/concentrations should be determined by the end user.
Format: Aqueous buffer solution
Formulation: 1 mg/ml in 10 mM PBS, pH 7.2, with 0.05% NaN3
Immunogen: 6x His synthetic peptide.
Purification: Protein A Affinity Chromatography
Storage Stability: Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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