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Applications: ChIP (4 µg/ChIP)
Assay Conditions: ChIP results obtained with the antibody directed against HP1α, β and γ
ChIP assays were performed using NIH3T3 cells and 4 µg of the antibody directed against HP1α, β and γ (Cat. # 25292). QPCR was performed on the immunoprecipitated DNA with optimized primer sets for the rDNA promoter and for a subtelomeric sequence of chromosome 19. Figure 1 shows the relative enrichment as compared to a no antibody negative control ChIP.Western blot analysis using the antibody directed against HP1α, β and γ
Western blot was performed on nuclear extracts from HeLa cells (20 µg) with the antibody against human HP1α, β and γ (Cat. # 25292) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the expected location of HP1α, HP1β and HP1γ is indicated on the right.Immunofluorescence using the antibody directed against HP1α, β and γ
HeLa cells were stained with the antibody against HP1α, β and γ (Cat. # 25292) and with DAPI. Cells were fixed with 4% formaldehyde for 10 min. and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HP1α, β, γ antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
Background: HP1 alpha, beta and gamma (UniProt/Swiss-Prot entry P45973, P83916 and Q13185) are components of heterochromatin. They recognize and bind histone H3 tails methylated at Lys-9, leading to epigenetic repression of transcription. HP1 alpha, beta and gamma also interact with lamin B receptor (LBR), thereby contributing to the association of heterochromatin with the inner nuclear membrane
Concentration: 50 µg/25 µl
Description: Polyclonal antibody raised in rabbit against human HP1β (Heterochromatin protein 1 homolog beta), using the full length recombinant GST tagged protein. The antibody also recognizes the HP1α and HP1γ isoforms.
Format: Aqueous buffer solution
Formulation: PBS containing 0.05% azide and 0.05% ProClin 300
Immunogen: recombinant protein
Purification: Protein G purified
Storage Stability: Store at -80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at -20°C for at least one month.
Uniprot: P45973, P83916, Q13185
Biosafety Level: Not applicable (BSL-1)
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