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Applications: WB (1:1000)
IF: (1:500)
Assay Conditions: Western blot analysis using the antibody directed against HP1α
Western blot was performed on nuclear extracts from HeLa cells (20 µg) with the antibody against human HP1α (Cat. No. 25291), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the expected location of HP1α, HP1β and HP1γ is indicated on the right. The Western blot analysis clearly demonstrates that the HP1α antibody specifically recognizes HP1α and not HP1β and HP1γ.Immunofluorescence using the antibody directed against HP1α
HeLa cells were stained with the antibody against HP1α (Cat. No. 25291) and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HP1α antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
Background: HP1 alpha (UniProt/Swiss-Prot entry P45973) is a component of heterochromatin. It recognizes and binds to histone H3 tails methylated at Lys-9, leading to epigenetic repression of transcription. HP1 alpha may also interact with lamin B receptor (LBR), thereby contributing to the association of the heterochromatin with the inner nuclear membrane. Further, HP1 alpha is involved in the formation of a functional kinetochore through interaction with MIS12 complex proteins.
Concentration: 50 µg/25 µl
Description: Polyclonal antibody raised in rabbit against human HP1 ? (Heterochromatin protein 1 homolog alpha), using the full length recombinant GST tagged protein
Format: Aqueous buffer solution
Formulation: PBS containing 0.05% azide and 0.05% ProClin 300
Immunogen: recombinant protein
Purification: Protein G purified
Storage Stability: Store at -80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at -20°C for at least one month.
Uniprot: P45973
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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