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Applications: ELISA (1:500)
Assay Conditions: Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human KDM1B (Cat. # 25299). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:14,250.Western blot analysis using the antibody directed against KDM1B
Nuclear extracts (25 µg) from K562 cells were analyzed by Western blot using the antibody against KDM1B (Cat. # 25299) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size 92 kDa) is indicated on the right; the marker (in kDa) is shown on the left.
Background: KDM1B (UniProtKB/Swiss-Prot entry Q8NB78), also known as LSD2, is a histone demethylase that demethylates lysine 4 of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. KDM1B is able to demethylate both mono- and di-methylated lysine 4 of histone H3, but has no effect on tri-methylated lysine 4, mono-, di- or tri-methylated lysine 9, mono-, di- or tri-methylated lysine 27, mono-, di- or tri-methylated lysine 36 of histone H3, or on mono-, di- or tri-methylated lysine 20 of histone H4. KDM1B is also required for de novo DNA methylation of a subset of imprinted genes during oogenesis. It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed.
Concentration: 100 µl
Description: Polyclonal antibody raised in rabbit against human KDM1B (lysine (K)-specific demethylase 1B) using two KLH-conjugated synthetic peptides containing a sequence from the N-terminal and the central region of the protein, respectively.
Format: Aqueous solution
Formulation: Whole antiserum from rabbit containing 0.05% azide
Immunogen: synthetic peptide
Purification: Whole serum
Storage Stability: Store at -80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at -20°C for at least one month.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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