Products from BPS Bioscience require a minimum order value above 400€
Applications: ELISA (1:1000 - 1:5000)
WB (1:1000)
Assay Conditions: Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of antibody directed against PADI4 (Cat. No. 25305). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1: 40,200.Western blot analysis using the antibody directed against PADI4
Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 40 µg) with the antibody against PADI4 (Cat. No. 25305), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right."
Background: Protein arginine deiminases catalyse the conversion of arginine residues in proteins to citrulline. PADI4 (UniProt/Swiss-Prot entry Q9UM07) catalyses the citrullination-deimination of Arg-8 and Arg-17 from histone H3 and Arg-3 from histone H4. This prevents their methylation by CARM1 and HRMT1L2/PRMT1, thereby repressing transcription. PADI4 also citrullinates EP300/P300 at Arg-2142, which favours its interaction with NCOA2/GRIP.
Concentration: 100 µl
Description: Polyclonal antibody raised in rabbit against human PADI4 (Protein-arginine deiminase type-4), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminus of the protein
Format: Aqueous solution
Formulation: Whole antiserum from rabbit containing 0.05% azide
Immunogen: synthetic peptide
Purification: Whole serum
Storage Stability: Store at -80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at -20°C for at least one month.
Uniprot: Q9UM07
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
English
Deutsch
