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Applications: ELISA (1:100 - 1:500)
WB (1:1000)
Assay Conditions: Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human PHF8 (Cat. # 25308), crude serum and flow-through in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:6,500. Western blot analysis using the antibody directed against PHF8
E14TG2A mouse embryonic stem cells were transfected with a conditional allele of PHF8. Nuclear extracts (135 µg) from wild type cells (WT) and from 2 clones (F1, B8) with an active and a targeted allele (F1ko, B8ko), respectively, were analyzed by Western blot using the antibody against PHF8 (Cat. # 25308). The antibody was diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. Western blot analysis was performed by Philipp Seibert, BIOTEC, Dept. of Genomics, TU Dresden, Tatzberg 47/49, 01307 Dresden.
Background: PHD finger protein 8 (UniProtKB/Swiss-Prot entry Q9UPP1) is a jumonji domain containing protein. Like other members of the jumonji family, PHF8 may therefore play a role in histone demethylation. Mutations in PHF8 lead to Siderius type X-linked mental retardation (MRXSSD), a mild to borderline type of mental retardation.
Concentration: 50 µg/39 µl
Description: Polyclonal antibody raised in rabbit against human PHF8 (PHD finger protein 8), using 3 different KLH-conjugated synthetic peptides.
Format: Aqueous buffer solution
Formulation: PBS containing 0.05% azide and 0.05% ProClin 300.
Immunogen: synthetic peptide
Purification: Affinity purified
Storage Stability: Store at -80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at -20°C for at least one month.
Uniprot: Q9UPP1
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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