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Products from BPS Bioscience require a minimum order value above 400€Application: Useful for studying expression of PSMA on the cell surface by flow cytometry
Assay Conditions: One million PSMA CHO cells (BPS Bioscience #79461) were blocked and stained with 1 µg of anti-PSMA antibody, FITC-labeled (BPS Bioscience #101977) for 30 minutes on ice, washed three times, and analyzed by flow cytometry. PSMA CHO cells (green) were compared to parental CHO-K1 cells (blue). The Y-axis represents the cell count, while the X-axis shows FITC intensity.
Background: PSMA (prostate-specific membrane antigen), also known as Folate hydrolase 1 or FOLH1, is an enzyme with folate hydrolase and peptidase activity. It is highly expressed in prostate cancer cells and is used as a prognostic indicator of prostate cancer. PSMA is used in the clinic as a target for PET (positron emission tomography) imaging in the detection and progression of prostate cancer, but radioactive analogs are used for the treatment of prostate cancer. It is also expressed in other tumor types and in a few normal tissues. PSMA is under extensive investigation as target for CAR-T cells and bi-specific antibodies-based cancer therapies.
Cross Reactivity: This antibody recognizes human PSMA. It has not been tested on other species.
Description: This human anti-PSMA antibody is a purified recombinant antibody, which is labeled with fluorescein isothiocyanate (FITC). This antibody has been tested by flow cytometry for binding to human PSMA on the surface of the PSMA CHO Recombinant Cell Line (BPS Bioscience #79461).
Format: Aqueous buffer solution.
Formulation: 8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, 0.09% Sodium Azide, 0.2% BSA, and up to 20% glycerol. May contain a protein stabilizer.
Host Cell Line: HEK293
Purification: Protein A affinity chromatography from HEK293 supernatants.
Purity: ≥90%
Storage Stability: At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE. The antibody solution should be stored undiluted between 2°C and 8°C.
Uniprot: Q04609
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
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