Anti-REA/PHB2 Antibody Picoband™ (monoclonal, 2B5)

Clone Number: 2B5

Immunogen: E.coli-derived human REA/PHB2 recombinant protein (Position: M1-K299).

Concentration: Adding 0.2 ml of distilled water =f 500 μg/ml.

Format: Lyophilized

Storage buffer: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Additional info: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.Add 0.2ml of distilled water will yield a concentration of 500ug/ml. Background: PHB2 (Prohibitin 2), also called Repressor of Estrogen Receptor Activity (REA), is a protein that in humans is encoded by the PHB2 gene. The International Radiation Hybrid Mapping Consortium mapped the PHB2 gene to chromosome 12. Montano et al. (1999) showed that REA enhanced the potency of a dominant-negative ER-alpha mutant and antiestrogens as suppressors of ER-alpha activity in Chinese hamster ovary cells. When coexpressed with wildtype ER-alpha or ER-beta (ESR2), REA suppressed activation of a reporter gene in a dose-dependent manner. REA had no effect on reporter activity in the absence of liganded ER, and it had no effect on the transcriptional activities of other hormone receptors. Mutation analysis showed that an N-terminal domain and a central domain of REA were required for its repressor activity. Subcellular Localization: Tissue Specificity:

• SKU: SANMON23787
• Manufacturer: Sanbio / Monosan
• Manufacturer SKU: MON23787
• Green Labware: No
• Package Unit: 100μg
• Quantity Unit: STK
• Reactivity: Human, Mouse (Murine), Rat (Rattus)
• Clonality: Monoclonal
• Application: Western Blotting, Flow Cytometry, Immunohistochemistry
• Isotype: IgG2b
• Host: Mouse