Products from BPS Bioscience require a minimum order value above 400€
Applications: WB, DB, ELISA, IP, ICC, WB.
Background: Red fluorescent protein cloned from Discosoma coral (DsRed or drFP583) is an important tool in biotechnology and cell biology as a spectrally distinct companion or substitute for the green fluorescent protein (GFP; from Aequorea jellyfish).
Clone Number: clone RF5R
Cross Reactivity: Does not cross-react with GFP (jellyfish).
Description: Affinity purified Anti-Red Fluorescent Protein (RFP), clone RF5R, Mouse IgG1. Recognizes native and denatured forms of RFP and its variants: tag-RFP, turbo-RFP, DeRed, mCherry, mOrange, etc. Purified from mouse ascites fluid. Applications: Western Blot (1:1000-3000), Dot Blot, ELISA, Immunocytochemistry (1:500-2000), Immunoprecipitation. Optimal dilutions/concentrations should be determined by the end user. Precited M.W. of RFP by Western blot is ~26 kDa.
Format: Aqueous buffer solution
Formulation: 1mg/ml in 10 mM PBS, pH 7.2, with 0.05% NaN3
Immunogen: Synthetic peptide conjugated to KLH, from the N terminal region of Sea anenome (Discosoma) RFP.
Purification: Protein A Affinity Chromatography
Storage Stability: Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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