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Applications: WB (1:1000)
Assay Conditions: Western blot analysis using the antibody directed against Set9
Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 20 µg) using the antibody against Set9 (cat. # 25312) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. A molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.
Background: Set9 (UniProtKB/Swiss-Prot entry Q8WTS6) is a histone methyltransferase which specifically methylates lysine 4 of histone H3 being a specific tag for epigenetic transcriptional activation. Further, methylation of histone H3K4 by Set9 precludes deacetylation of H3 by the NuRD complex and impairs Suv39h1-mediated methylation of H3K9. Set9 also methylates the tumor suppressor p53 at lysine 372, thereby enhancing its stability. It contains a SET domain but lacks the pre- and post-SET domains.
Concentration: 50 µg/25 µl
Description: Polyclonal antibody raised in rabbit against human Set9 (SET domain containing (lysine methyltransferase) 9), using a recombinant protein.
Format: Aqueous buffer solution
Formulation: PBS including 0.05% azide and 0.05% ProClin 300
Immunogen: recombinant protein
Purification: Affinity purified
Storage Stability: Store at -80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at -20°C for at least one month.
Uniprot: Q8WTS6
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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