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Applications: WB (1:1000 - 1:1
200)
IP (5 µg/mg of RIPA lysate)
Assay Conditions: Western blot analysis using the monoclonal antibody directed against TET2
Figure 1A. Whole cell extracts from HL60 and NB4 cells (40 µg) were analyzed by Western blot using the antibody against TET2 (Cat. # 25320), diluted 1:2,000 (lane 1 and 2) or 1:1,000 (lane 3 and 4) in PBS containing 10% milk. The position of the protein of interest (expected MW 224 kDa) is indicated on the right; the marker (in kDa) is shown on the left.
Figure 1B. Western blot on mouse E14 ES cells. The antibody was used at a dilution of 1:1,000.Immunoprecipitation using the monoclonal antibody directed against TET2
IP was performed on 250 µg HL60 RIPA cell lysate using the antibody against TET2 (Cat. # 25320) (lane 3) or an IgG negative control (lane 2). The samples were analyzed by Western blot analysis as described above. The input sample (25 µg RIPA lysate) was used as a positive control (lane 1).
Background: TET2 (UniProt/Swiss-Prot entry Q6N021) is a methylcytosine dioxygenase that catalyzes the conversion of 5-methylcytosine to 5-hydroxymethylcytosine (5-hmC). 5-hmC has been recently discovered in mammalian DNA and is abundant in Purkinje neurons, granule cells, embryonic stem cells, and brain tissue, especially in areas that are associated with higher cognitive function. Although its precise role has still to be shown, recent studies indicate that 5-hmC plays important roles distinct from 5-mC. Early evidence suggests that 5-hmC may represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine. Mutations in TET2 have been associated with myeloproliferative diseases such as essential thrombocythemia, polycythemia vera and primary myelofibrosis.
Concentration: 50 µg/50 µl
Description: Monoclonal antibody raised in mouse against the a recombinant protein containing the N-terminal 300 amino acids of human TET2 (tet oncogene family member 2).
Format: Aqueous buffer solution
Formulation: PBS containing 0.05% azide
Immunogen: recombinant protein
Purification: Protein G purified
Storage Stability: Store at -80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at -20°C for at least one month.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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