Antigen Long Description: The original antibody was generated by immunizing BALB/c mice with tetracycline hydrochloride-BSA as an immunogen. The cDNA obtained from total mRNA of the immunized mouse splenocytes was used as a template to amplify the variable domain genes and generate an scFv phage display library. Later on the antibody was selected by panning this library against tetracycline-ovalbumin conjugate.