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Products from BPS Bioscience require a minimum order value above 400€Application: Useful for labeling cells expressing TIM-3 for flow cytometry and immunofluorescence microscopy.
Assay Conditions: Tim3-biotin (BPS Bioscience #7123) was titrated from 0 to 300 nM with a 3-fold dilution series in presence of 0 and 1000 ng/well of immobilized anti-Tim3 (BPS Bioscience #79098) and incubated for 1 hour at RT. Binding was detected with Streptavidin-HRP (BPS Bioscience #79742) and ELISA ECL Substrate (BPS Bioscience #79670).
Cross Reactivity: This antibody has not been tested for cross-reactivity with any other species.
Description: Recombinant human anti-Tim3 antibody recognizing human TIM3 protein.
Format: Aqueous solution
Formulation: 8 mM Na Phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl and 20% glycerol.
Purification: Protein A affinity chromatography from HEK293 supernatants.
Storage Stability: Stable for at least 12 months at -80°C. Avoid freeze/thaw cycles. Store diluted antibody with carrier protein (BSA 0.1 - 0.5%) at 4°C, stable for 2 weeks.
Supplied As: Aqueous buffer solution.
Uniprot: Q8TDQ0
Biosafety Level: Not applicable (BSL-1)
References: 1.Zhu, C. et al., Nat. Immunol. 2005; 6:1245-1252.2.Anderson, A.C. et al., Science. 2007; 318(5853):1141-1143.
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