Anti-Urease alpha subunit (HpU-2)

Anti-Urease alpha subunit [HpU-2], Recombinant, IgG2a kappa, Mouse

CloneID: HpU-2

Heavy Chain modification: Fc Silent™

Antigen Long Description: HpU-2 was prepared by subcutaneous injection of purified H. pylori urease into BALB/c mice.

Buffer Composition: PBS only.

Chimeric Use Statement: This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.

Uniprot Accession No.: P14916

Specificity Statement: HpU-2 binds specifically to H. pylori at the epitope with sequence SVELIDIGGNRRIFGFNALVDR - the sequence is part of a loop near to the C-terminus of the alpha subunit of urease (the beta subunit possesses the active site). HpU-18 competes with HpU-2 for binding to this epitope. H. pylori is a gram negative spiral bacterium and causes numerous gastroduodenal diseases. It expresses large amounts of urease when present in the human stomach; urease is key for the survival and pathogenesis of the bacteria as it neutralizes the acidity in the stomach by hydrolysis of urea into ammonia.

Application Notes (Clone): HpU-2 results in ~82% inhibition (allosteric inhibition) of urease activity and the inhibitory activity is increased to 87% when HpU-2 is used in combination with HpU-19. HpU-2 has been shown be unsuccessful for use in IHC of formalin-fixed sections of H. pylori-infected human cells. HpU-2 can also be used in SPR and ELISA studies.

• SKU: ABAAb00764-2.3-BT
• Manufacturer: Absolute Antibody
• Manufacturer SKU: Ab00764-2.3-BT
• Package Unit: 1 mg
• Quantity Unit: STK
• Reactivity: Various species
• Clonality: Recombinant
• Application: ELISA, Super-Resolution Microscopy
• Isotype: IgG2a kappa
• Host: Mouse