CloneID: B5
Heavy Chain modification: His-Tagged
Antigen Long Description: B5 was isolated from a phage display library constructed using spleenic mRNA from non-immunized mice after 3 rounds of panning.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This reformatted mouse antibody was made using the variable domain sequences of the original Mouse scFv format, for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: Q90YN8
Specificity Statement: B5 recognises a linear epitope of Vtg at the N-terminal region of the protein from zebrafish, Chinese rare minnow, common carpand crucian carp. Vtg is the phospholipoglycoprotein precursor to the major egg yolk protein which is synthesized in the liver in response to endogenous estrogen in females. Vtg synthesis in males can be used as a biomarker for exposure to estrogen and estrogen mimics in males where Vtg levels are normally low but can be increased upon exposure.
Application Notes (Clone): B5 scFv can be used in WB and ELISA studies.
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