Application Note: WB: 0.25-0.5 μg/ml. FACS: 1-3 μg/1x10⁶ cells. ELISA: 0.1-0.5 μg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Calculated MW: 142
Form: Liquid
Buffer (with preservative): 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, no preservatives.
Concentration: 0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Background: This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Uniprot ID: O14727
Antigen Species: Human
Immunogen: E.coli-derived human APAF1 recombinant protein (Position: M1-E1248)
Purification: Purified by antigen-affinity chromatography
Conjugation: Unconjugated
Full Name: apoptotic peptidase activating factor 1
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