Target: Aquaporin 2.
Conjugate: ATTO 390.
Product Type: Polyclonal.
Immunogen: Produced against the C-terminal peptide (Sequence N-CLKGLEPDTDWEEREVRRRQ) of rat aquaporin 2.
Swiss-Prot: P34080.
Purification: Protein A Purified.
Storage Buffer: PBS, 50% glycerol, 0.09% sodium azide.
Concentration: 1 mg/ml.
Specificity: Detects ~28.8kDa. May detect larger glycosylated bands ~35-50kDa.
Cellular Localization: Cell Membrane, Apical Cell Membrane, Basolateral Cell Membrane, Cytoplasmic Vesicle, Cytoplasmic Vesicle Membrane, Golgi Apparatus.
Scientific Background: Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins (1, 2). Aquaporin 2 is the vasopressin-regulated water channel of the apical membrane of collecting duct cells. It is located in kidney epithelial cells and usually lies dormant in intracellular vesicle membranes. When it is needed vasopressin binds to the cell surface vasopressin receptor, activating a signaling pathway that cause AQP2 containing vesicles to fuse with the plasma membrane so the AQP2 can be used by the cell (3). Defects in AQP2 area cause of an autosomal dominant form of nephrogenic diabetes insipidus (NDI) (4).
References: 1. Gonen T., Walz T. (2006) Q. Rev. Biophys. 39(4): 361-396.2. Knepper M.A. (1994) Proc Natl. Acad Sci. USA. 91(14): 6255-6258.3. Lodish H.F. (2008) Molecular Cell Biology. New York: W.H. Freeman. Print. 445.4. www.vivo.colostate.edu/hbooks/molecules/aquaporins.html.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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