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Encompassing Amino Acids: 282-606 (end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Reaction was performed in a kinase buffer (40 mM Tris, 10 mM MgCl2) containing 5 µg inactive MEK1 (BPS #40075), 1 µM ATP, and 4 - 8 ng/µl aRAF for 30 minutes at 30°C. The amount of ATP transferred was measured using a Kinase-Glo® Plus Assay Kit (Promega Corporation, Madison, WI).
Description: Human v-raf murine sarcoma 3611 viral oncogene homolog, transcript variant 1 (A-RAF1), (GenBank Accession No. NM_001654), a.a. 282-606 (end), containing mutations YY301-302DD, with N-terminal GST-tag and C-terminal His-tag, MW=63 kDa, expressed in an Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol and 3 mM DTT.
Genbank: NM_001654
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag, C-terminal His-tag
Uniprot: P10398
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Yokoyama,T. et al. J. Cell Biol. 177 (5), 781- 793 (2007).
2. Ewing,R.M. et al. Mol. Syst. Biol. 3, 89 (2007).
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