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Applications: Monitor Nrf2 antioxidant response pathway activity.
Screen for activators or inhibitors of the Nrf2 antioxidant response pathway.
Background: The Nrf2 antioxidant response pathway plays an important role in cellular antioxidant defense. Nrf2, a basic leucine zipper transcription factor, induces the expression of antioxidant and phase II enzymes by binding to the ARE (antioxidant response element) region of the gene promoter. Under basal conditions, Nrf2 is retained in the cytosol by binding to the cytoskeletal protein Keap1. Upon exposure to oxidative stress or otherARE activators, Nrf2 is released from Keap1 and translocates to the nucleus, where it can bind to the ARE, leading to the expression of antioxidant and phase II enzymes that protect the cell from oxidative damage.
Biological Activity: This cell line is validated for the response to the stimulation of tert-butylhydroquinone and sulforaphane.
Description: The ARE Reporter - Hep G2 cell line is designed to monitor Nrf2 antioxidant response pathway. The cell contains a firefly luciferase gene under the control of ARE (antioxidant response element) stably integrated into Hep G2 cells.
Format: aqueous solution containing DMSO
Host Cell Line: HepG2
Mycoplasma Testing: The cell line has been screened using the PCR-based Venor™GeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.
Storage Stability: Store in liquid nitrogen immediately upon receipt.
Supplied As: Each vial contains ~2 X 10^6 cells in 1 ml of 10% DMSO.
Uniprot: Q16236
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-1
References: 1. Lee, JM et al. (2004) An important role of Nrf2-ARE pathway in the cellular defense mechanism. J Biochem Mol Biol. 37(2): 139-143.
2. Dinkova-Kostova, AT et al. (2002) Direct evidence that sulfhydryl groups of Keap1 are the sensors regulating induction of phase 2 enzymes that protectagainst carcinogens and oxidants. Proc Natl Acad Sci U S A. 99(18): 11908-11913.
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